Dcfh staining
WebAug 17, 2024 · ROS level was assessed by DCFH 2-DA staining and presented as a relative value to that of the control. (B) Lung cancer cells were pretreated with NAC (1 mM) for 30 min in the presence or absence of AA (50 μM) for 24 h. Cell viability was determined using the MTT assay. WebApr 13, 2024 · DCFH-DA was used as the probe to determine the intracellular ROS generation ability in MCF-7 cells ( Zhang et al., 2014; Karaoğlu et al., 2024 ). MCF-7 cells were seeded in four-well plates at a density of 1.0 × 10 4 cells per dish for 24 h. The cells were co-incubated with CBT-SiPc (3.0 μM) for 10 h.
Dcfh staining
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http://content.dcf.ks.gov/EES/KEESM/Appendix/HEALTH_AND_SAFETY_STANDARDS.pdf WebApr 4, 2024 · Dynamic light scattering analysis showed a smaller hydrodynamic diameter of nanoparticles in DMEM (containing 10% FBS) than in phosphate-buffered saline (Table S1). In addition, the SPIONs and mSPIONs showed negative zeta potential (Table S1). The mSPIONs were stable in DMEM culture medium (Figure S2).
WebThe cell-permeant 2',7'-dichlorodihydrofluorescein diacetate (H 2 DCFDA) (also known as dichlorofluores cin diacetate) is a chemically reduced form of fluorescein used as an … WebKeyword:'dcfh-da' Showing 1-2 of 2 results for "dcfh-da" within Products. Products Genes Papers Technical Documents Site Content Chromatograms. Filter & Sort. All Photos (1) 2′,7′-Dichlorofluorescein diacetate. Empirical Formula (Hill Notation): C 24 H 14 Cl 2 O 7. CAS No.: 2044-85-1. Molecular Weight: 485.27. Beilstein No.: 68488. Compare
WebIn the following protocol, we use DCFH-DA dye to stain reactive oxygen species in the colorectal cancer cells. - To stain the cells, remove the drug-containing medium and … WebA. Paige Davis Volk, Jessica G. Moreland, in Methods in Enzymology, 2014 5.2.2 DCFH. DCFH (2,7-dichlorodihydrofluorescein) is the most widely used fluorescent probe for the …
WebDescription: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Wash cultured cells with PBS for three times. 2. Treat testing cells with H2DCFDA in culture medium for …
WebMar 24, 2024 · b Representative images of hematoxylin-eosin (H&E), WGA, Masson’s trichrome, Sirius red staining in cardiac tissues. ... d Representative images of DHE and DCFH-DA staining in the cardiac tissues. rockers for sunroomWebApr 30, 2024 · dcfh-da（二氯二氢荧光素-乙酰乙酸酯）可自由透过活细胞膜进入细胞内，并被细胞内的酯酶水解，形成dcfh，dcfh无荧光且不能通透细胞膜，其可以被细胞内的活性氧（ros）氧化生成有荧光的dcf，使用流式细胞仪或荧光显微镜检测和观察活细胞内dcf的荧光 … rockers from the land of reggaeWebMar 21, 2014 · The DCFDA assay protocol is based on the diffusion of DCFDA / H2DCFDA / DCFH-DA / DCFH into the cell. It is then … rockers for short peopleWebApr 3, 2024 · Materials and methods. ICR mice with CCl 4-induced hepatic fibrosis were supplemented with Sch B (40 mg/kg) for 30 d and LX2 cells were treated with Sch B (5, 10 and 20 μM) for 24 h.Cellular senescence was assessed by senescence-related indicators senescence-associated β-galactosidase (SA-β-gal) activity and the expression of p16, … rockers for tall peopleWebJun 6, 2024 · DCFH-DA staining is a simple and cost-effective way to detect ROS in cells. It can be used to measure ROS generation after chemical treatment or genetic … rockers from the land of reggae i mo jahWebDichlorodihydrofluorescein diacetate (DCFH-DA) is a reduced, cell permeable ester that is hydrolyzed in the intracellular environment to dihydroxy-DCFH. From: Free Radical … otc 5213taWeb2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation. Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative ... rockers footwear