WebPrussian blue staining for iron shows a marked increase in iron deposition, mainly in the reticuloendothelial cells. 513 Some patients have minimal or no deposition of iron. 515,526 There is no correlation between the extent of liver damage and iron deposition. 515 Electron microscopic studies show swollen hepatocytes filled with glycogen. The ... WebPerls' Prussian blue stain of a liver biopsy from a patient with hereditary hemochromatosis. Left panel: Low power view shows intense iron staining of hepatocytes. The blue-stained iron deposits typically start at the periphery of the liver lobule and extend centrally.
The use of special stains in liver biopsy interpretation ... - PubMed
Potassium ferrocyanide in the staining solution combines with the ferric iron forming the Prussian blue pigment. The addition of hydrochloric acid increases the availability of iron within the tissue for reaction with the potassium ferrocyanide. The chemical reaction for the conversion of iron to Prussian blue is provided as … See more In histology, histopathology, and clinical pathology, Perls Prussian blue is a commonly used method to detect the presence of iron in tissue or cell samples. Perls Prussian Blue derives its name from the German … See more Perls did not publish a detailed procedure other than indicating a dilute potassium ferrocyanide solution was applied to the tissue followed by hydrochloric acid. Ferric iron deposits in tissue (present mostly as ferric iron within the storage protein ferritin) then react with the … See more Perls's method is used to indicate "non-heme" iron in tissues such as ferritin and hemosiderin, the procedure does not stain iron that is bound to See more WebAug 4, 2024 · The paraffin-embedded liver was sectioned into 4.5-micron slices, followed by deparaffinisation and rehydration. Prussian blue was used to stain iron histologically, while haematoxylin was used to stain nuclei. The grading of iron deposition brian copenhaver
Prussian blue staining images of (A) brain, (B) heart, (C) lung, (D ...
WebIron Stain Kit (Prussian Blue stain) ab150674 is intended for use in the detection of ferric iron in tissues, blood smears, or bone marrow smears. Ferric iron is normally found in … WebAug 30, 2024 · Perls iron stain in a normal liver is negative (no blue granules). Figure 8. Perls iron showing increased iron deposition (blue color) in a patient with … WebExample of the use of special stains and counterstains. Prussian blue i ron stain kit staining iron (blue) in liver tissue. Nuclei and background were counterstained with nuclear fast red. Chromogenic counterstains Chromogenic counterstains are used when the primary antibody is visualized using HRP or alkaline phospatase combined with DAB, AEC, or … brian copper fire tees